Development of an Astrocyte Hemichannel Blocker to Delay Spatial and Temporal Progression in ALS

Abstract

The mechanisms by which disease progresses in ALS, both anatomically and temporally, is unknown. Halting or slowing disease progression after onset, when patients are typically diagnosed, would offer enormous practical therapeutic potential. A consistent theme in our understanding of disease progression, after onset, in ALS suggests that astrocytes play a role in this progression. Our most recent work, implicates the astrocyte connexin, Cx43, as a mediator of MN toxicity using in vitro mouse ALS modeling. We hypothesize that the network of astrocytic hemichannels (HC), composed of connexin 43, may modulate the spatial and temporal progression of disease which ultimately results in MN death or dysfunction. Therefore, modulatingCx43 HC may be an excellent target for an ALS therapeutic. Tonabersat is a drug that has been shown to block Cx43 hemichannels in several studies and this hypothesis provides the foundation for this program. We will use human induced pluripotent stem cells from ALS patients along with animal models of ALS to study Cx43 HC mediated toxicity in ALS and to examine the ability of Cx43 HC blockers to block neurotoxicity in these models.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2021
Accession Number
AD1153328

Entities

People

  • Nicholas J Maragakis

Organizations

  • Johns Hopkins University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Animal Structures
  • Astrocytes
  • Availability
  • Biological Markers
  • Biomedical Research
  • Cells
  • Culture Techniques
  • Disease Attributes
  • Diseases And Disorders
  • Maryland
  • Motor Neurons
  • Neurons
  • Side Effects
  • Spinal Cord
  • Stem Cells
  • Targets
  • Technology Transfer
  • Therapy
  • Toxicity

Fields of Study

  • Biology

Readers

  • Immunology and Pathology
  • Neuroscience

Technology Areas

  • Biotechnology