Understanding the 'Mission Versatility' of Membrane Proteins via Nanoscopic Imaging

Abstract

The goal of this AFOSR Young Investigator Program grant is to investigate the nanoscale structural and functional dynamics of membrane proteins in their native liquid environment using liquid phase transmission electron microscopy (TEM). Over the project time period, we optimized the self-assembly and purification of nanodiscsmembrane proteins in a single-particle form amenable to TEMobservationcontaining the protein cytochrome p450 2J2 (CYP-2J2) and then developed a graphene liquid sandwich to encapsulate CYP- 2J2 nanodiscs in their native buffer conditions for imaging.

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Document Details

Document Type
Technical Report
Publication Date
Sep 30, 2021
Accession Number
AD1155537

Entities

People

  • Qian Chen

Organizations

  • University of Illinois Urbana–Champaign

Tags

Communities of Interest

  • Advanced Electronics

DTIC Thesaurus Topics

  • Boundaries
  • Chemical Analysis
  • Chemistry
  • Dynamics
  • Electron Microscopy
  • Engineering
  • Lipids
  • Liquid Chromatography
  • Liquid Phases
  • Membrane Proteins
  • Microscopy
  • Molecular Dynamics
  • Proteins
  • Self Assembly
  • Statistical Mechanics
  • Transmission Electron Microscopy
  • Universities

Fields of Study

  • Physics

Readers

  • Molecular and Cellular Biochemistry
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Microelectronics
  • Microelectronics - Graphene