Direct Imaging of Modulatory Neurotransmitters Using Synthetic Nanosensors to Understand and Treat Parkinson's Disease

Abstract

Nanosensors for the optical detection of dopamine in brain slice has been validated in wildtype C57/C6 mice as well as a Parkinson's model mouse. Using nanosensors, endogenous dopamine release in striatal brain slice is triggered by electrical stimulation and quantified using microscopy. We have established the workflow for this procedure and have prepared a population of Parkinson's and wildtype mice to image in the coming quarter. We have confirmed that the method can quantitatively distinguish dopamine release between sub-regions of the striatum, a critical step in confirming the method for use in Parkinson's studies. COVID-19 shutdowns stalled research starting in Mar. Research facilities are reopening and the project is expected to resume this quarter.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
May 01, 2021
Accession Number
AD1155695

Entities

People

  • Jackson Del Bonis-o'donnell

Organizations

  • University of California Regents

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biomedical Research
  • Brain
  • California
  • Carbon Nanotubes
  • Covid-19
  • Data Analysis
  • Detectors
  • Diseases And Disorders
  • Dopamine
  • Governments
  • Medical Personnel
  • Neurons
  • Parkinson'S Disease
  • Public Health
  • Research Facilities
  • Students
  • Therapy

Readers

  • Clinical Trial Research.
  • Neurodegenerative Parkinson's Disease and Rickettsial Disease handbook, including the data level of dopamine, BC, neurons, and PD.
  • Neuroscience