A SARS-CoV-2 Spike Ferritin Nanoparticle Vaccine Protects Against Heterologous Challenge with B.1.1.7 and B.1.351 Virus Variants in Syrian Golden Hamsters
Abstract
The emergence of SARS-CoV-2 variants of concern (VOC) requires adequate coverage of vaccine protection. We evaluated whether a spike ferritin nanoparticle vaccine (SpFN), adjuvanted with the Army Liposomal Formulation QS21 (ALFQ), conferred protection against the B.1.1.7and B.1.351 VOCs in Syrian golden hamsters. SpFN-ALFQ was administered as either single or double-vaccination (0 and 4 week) regimens, using a high (10 microgram) or low (0.2 microgram) immunogen dose. Animals were intranasally challenged at week 11. Binding antibody responses were comparable between high- and low-dose groups. Neutralizing antibody titers were equivalent against WA1, B.1.1.7, and B.1.351 variants following two high dose two vaccinations. SpFN57 ALFQ vaccination protected against SARS-CoV-2-induced disease and viral replication following intranasal B.1.1.7 or B.1.351 challenge, as evidenced by reduced weight loss, lung pathology, and lung and nasal turbinate viral burden. These data support the development of SpFN-ALFQ as a broadly protective, next-generation SARS-CoV-2 vaccine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 16, 2021
- Accession Number
- AD1156227
Entities
People
- Adrienne Winn
- Agnes Hajduczk
- Akshaya Ganesh
- Alexander Q Anderson
- Amy R. Henry
- Andrew S. Pekosz
- Caitlin Kuklis
- Caroline E. Peterson
- Daniel C. Douek
- Diane L Bolton
- Dominic Paquin-proulx
- Elham B. Mokhtari
- Eli A. Boritz
- Elizabeth J. Martinez
- Erica K. Barkei
- Gary R Matyas
- Gregory D. Gromowski
- Hannah Grove
- Heather Hernandez
- Holly Hack
- Inès Lakhal-Naouar
- Isabella Swafford
- James F. Wood
- Janice Anderson
- Jeffrey R. Currier
- Jeffrey W. Froude
- Kamil Radzyminski
- Kathryn M. Wuertz
- Kayvon Modjarrad
- Linda L. Jagodzinski
- Lindsay Sandrine
- M. G. Joyce
- Maciel Porto
- Mangala Rao
- Mark G. Lewis
- Matthew Gagne
- Mehul Suthar
- Michelle Zemil
- Ming Dong
- Nelson Michael
- Paul V. Thomas
- Prakriti Mudvari
- Rafael De La Barrera
- Rajeshwer S. Sankhala
- Robert A Seder
- Sandhya Vasan
- Sandrine Soman
- Sharon P. Daye
- Sheila A. Peel
- Shelly J. Krebs
- Stasya Zarling
- Swagata Kar
- Victoria R. Polonis
- Vincent Dussupt
- Wei-hung Chen
- Xiankun Zeng
Organizations
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Walter Reed Army Institute of Research