Effects of Ionizing Radiation (IR) on Myocardial DNA Methylation Profiles in Relation to Cardiomyopathy in a Nonhuman Primate Model

Abstract

Specific Aims are to determine 1: The effects of radiation exposure on the myocardial DNA methylome; 2: Relationships between myocardial methylation profiles and cardiomyopathy(myocardial fibrosis, heart structural and functional characteristics; and 3: Relationships between myocardial DNA methylation profiles and myocardial transcriptional profiles, the latter supported by another mechanism. Progress: Aim 1: We have completed the tissue distribution and processing, DNA isolation and characterization, and DNA methylation analysis work using Illumina Infinium(registered trademark) Methylation EPIC BeadChips. Aim 2: Cardiac phenotyping completed. Aim 3: We have completed analysis of the transcriptomes of these same tissues using RNAseq as well as Nanostring technologies which will be integrated with the DNA methylation results. Some of this work was presented at the 2021 Radiation Research Society Meeting. Analyses continue and will be incorporated into a final manuscript which will represent significant body of work.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2021
Accession Number
AD1156677

Entities

People

  • Thomas C. Register

Organizations

  • Wake Forest University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Cardiomyopathies
  • Cardiovascular Diseases
  • Computational Biology
  • Department Of Defense
  • Diseases And Disorders
  • Gastroenteritis
  • Gene Expression
  • Health
  • Heart
  • Heart Diseases
  • Ionizing Radiation
  • Medical Personnel
  • Radiation
  • Statistical Analysis
  • Students

Fields of Study

  • Biology

Readers

  • Medical Imaging.
  • Molecular Genetics
  • Oncology and Biomarker-Based Cancer Detection.