Peptidylarginine Deiminase 2 and Citrullination of IgG in Immunity and Rheumatoid Arthritis

Abstract

The purpose of this application is to identify mechanisms by which peptidylarginine deiminases (PADs)and citrullination regulate antibodies in immunity and rheumatoid arthritis. To this end, this project will (1)determine how PADs and IgG citrullination regulate a normal antibody response to immunization and normal antibody-based immunity to influenza, (2) identify how PADs and citrullinated IgG pathologically contribute to rheumatoid arthritis, and (3) determine if smoking increases IgG citrullination leading to autoimmune antibodies in genetically susceptible people. To date, we have discovered that PAD2 is not required for plasma cell numbers or arthritis severity in collagen-induced murine arthritis, but is required for anti-collagen IgG levels. Further, PAD2 is required for some antibodies formed in response to murine influenza. Experiments are ongoing to further understand these findings as well as to evaluate citrullination in autoimmune antibodies in humans.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2021
Accession Number
AD1159622

Entities

People

  • Miriam A. Shelef

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Arthritis
  • Biomedical Research
  • Blood
  • Cells
  • Collagen
  • Covid-19
  • Data Analysis
  • Health Services
  • Immunity
  • Immunization
  • Influenza
  • Lymphocytes
  • Mass Spectrometry
  • Medical Personnel
  • Proteins
  • Sars
  • Spectrometry
  • Statistical Analysis
  • Students
  • Therapy
  • Viruses

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology and Pathology
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech