Development of Diagnostic Tools for Metastatic Melanoma via Imaging of Heparanase Activity

Abstract

Imaging the activity of heparanase is a promising approach to visualizing aggressive melanoma. However, current detection methods of heparanase activity lack specificity and sensitivity and no probe currently exists for in vivo imaging. In this research period, we have successfully developed key chemical groups to make the heparanase activatable probes. The synthesis of the final PET tracer is ongoing. We have successfully produced human recombinant heparanase using mammalian cells to facilitate the examination of our synthetic molecular probes. We have also screened through various human melanoma cell lines to identify the cell lines with the highest heparanase expression level. When we have efficient heparanase probes in hand, we will evaluate the effects of heparanase activity on oncogenic properties of melanoma cell lines, and on cell migration and invasion.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2021
Accession Number
AD1160947

Entities

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  • Kelton Schleyer
  • Lina Cui
  • Zixin Chen

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  • University of Florida

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  • Biomedical

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