Aerosol Delivery of CPZEN-45 for Treatment of Nontuberculous Mycobacterial (NTMs) Infections

Abstract

This DOD Therapeutic Development Award is focused on a new antibiotic, CPZEN-45, discovered by our team for treatment of non-tuberculous mycobacterial infections (NTM) in patients with chronic obstructive pulmonary disease (COPD). We have two major objectives: Objective 1-To optimize fermentation and scale-up of manufacturing processes for high yield of CPZEN-45, including spray dried CPZEN-45. Objective 2- To further define and characterize in vitro efficacy of CPZEN-45 against additional species of NTMs recently isolated from VA patients with COPD. Specifically, our objective is to develop an efficacious regimen for COPD patients by screening multiple CPZEN-45 combinations with standard NTM compounds in human THP-1 cells using a checkerboard assay. Synergy measurement by checkerboard analysis will be used to determine the impact on potency of the combination of antibiotics in comparison to their individual activities. The optimized synergistic regimens will then be tested in COPD mouse and guinea pig efficacy models.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2021
Accession Number
AD1162367

Entities

People

  • Darrick Carter
  • Gail Cassell

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Biological Sciences
  • Biomedical Research
  • Cells
  • Cellular Structures
  • Chemistry
  • Department Of Defense
  • Diseases And Disorders
  • Electronic Mail
  • Health Services
  • Infection
  • Infectious Diseases
  • Lung Diseases
  • Manufacturing
  • Medical Personnel
  • North Carolina
  • Production
  • Rodents
  • Wound Infections

Fields of Study

  • Medicine

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