Local Mesenchymal Stem Cell Therapy for the Ex Vivo Preservation of a Porcine Vascularized Composite Allograft with 24-Hours Normothermic Machine Perfusion
Abstract
Advances in both body armor and trauma life support have increased the survival rate of combat casualties. However, there remains vast limitations in extremity war injuries and limb salvage. As a result, the rise of vascularized composite allotransplantation (VCA)has increasingly gained traction. Since preservation research is still in its infancy, multiple factors regarding cold ischemia time and perfusion following injury through surgical intervention need to be established. Machine perfusion has become a military area of interest for organ preservation in transplantation since limitations involving (1) prolonged travel (i.e. geographical distance) and (2) time before surgical intervention are the norm in combat related injuries. Additionally, the introduction of mesenchymal stem cell (MSC)-based therapy has shown promise in VCA studies by attributing improved immunomodulation and regenerative properties. Previous VCA research models, using University of Wisconsin (UW) preservation solution under hypothermic conditions and the use of whole blood in normothermic conditions have been performed. However, limited data exist addressing the use of MSCs during an extended preservation phase of an ex vivo VCA study. The purpose of this study is to demonstrate the efficacy of combining local MSC infusion with fresh whole blood in an ex vivo porcine free flap VCA model utilizing normothermic machine perfusion (NMP) for 24 hours. We hypothesized that local intravascular infusion of MSCs during NMP will facilitate the attachment of MSCs to the VCA vasculature and lead to tissue repair as well as aid in the prevention of additional cell injury; reducing inflammatory responses during the ex vivo preservation phase.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 16, 2022
- Accession Number
- AD1163669
Entities
People
- Alejandro Bernal
- Cody Hinshaw
- Diandra Wood
- Erik K. Weitzel
- Jennifer Hinshaw
- Jian Ling
- Jorge J. Pena
- Kreg A. Zimmern
- Margaux M. Salas
- Nick Mcmahon
- Wensheng Zhang
Organizations
- 59th Medical Wing