Prevention of the Post-Traumatic Fibrotic Response in Joints: A Critical Preclinical Evaluation of an Antifibrotic Antibody

Abstract

We successfully tested in vivo the validity of the concept that blocking the formation of fibrotic scars limits post-traumatic joint stiffness. Specifically, we rationally engineered a therapeutic anti-collagen I antibody (referred to as ACA) that targets collagen I molecules and prevents their participation in scar formation. In the course of our previously-funded research, we validated our approach in a relevant animal model of post-traumatic joint stiffness. Here, we plan to move our research from the proof-of-concept phase closer toward clinical tests. We have reached important milestones that define the course of our proposed study. These milestones include: (i) verifying fibrotic scarring as a valid target to limit post-traumatic joint stiffness; (ii) engineering a clinically-relevant form of the ACA to inhibit fibrotic scarring; and (iii) validating in vivo the efficacy of the ACA to limit post-traumatic joint stiffness. Here, we will continue our preclinical research to achieve the following goals: (i) to define the concentration-dependent safety and efficacy of our ACA; (ii) to determine the effects of the ACA on the healing of joint tissues; and (iii) to define clinically-relevant biomarkers to monitor the effects of the ACA-based treatment of injured joints. Achieving these goals will provide a launching platform for future clinical tests. Objective/Hypothesis. The main objective of this study is to limit post-traumatic joint stiffness in military personnel by employing an antibody-based blocker of fibrotic scarring. We hypothesize that applying this therapeutic antibody will reduce post-traumatic joint stiffness without causing any significant unwanted side effects.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2021
Accession Number
AD1164044

Entities

People

  • Andrzej Fertala

Organizations

  • Thomas Jefferson University

Tags

DTIC Thesaurus Topics

  • Anesthesia
  • Blood
  • Cartilage
  • Cells
  • Connective Tissue
  • Diseases And Disorders
  • Engineering
  • Health Services
  • Joints (Anatomy)
  • Mechanical Properties
  • Medical Personnel
  • Molecules
  • Musculoskeletal System
  • Orthopedics
  • Rodents
  • Surgery
  • Tissues

Fields of Study

  • Medicine

Readers

  • Immunology and Pathology
  • Naval Engineering and Maritime Security
  • Oncology