Subnormothermic Ex Situ Limb Perfusion Using Red Blood Cells and Plasma: Critical Component Analysis
Abstract
The long-term objective of this application is to keep vascularized composite tissues viable at or beyond 48 hours using ex-vivo perfusion systems. In this project, we used rat hind limb allograft transplantation model along with a miniaturized version of our custom-made ex-vivo perfusion system and tested red blood cell based perfusate solutions. Under SA1, we tested the efficacy of three plasma components in maintaining on muscle force generation on limb allografts after 8 hours of SNT ex-vivo perfusion/transplantation. These components (full, high molecular weight, and low molecular weight plasma) mixed with red blood cells were used to perfuse limbs, which were then transplanted to another recipient orthotopically to observe limb survival as well as the long-term outcomes of muscle and nerve regeneration 3 months following trasnplantation. Outcome measures included analysis of the EMG data, muscle and nerve histology, and proteomic analysis of the perfusate. At the end of first year, we completed all transplantation surgeries in control and experimental groups. In addition, we also completed EMG analysis of all control groups, and 80% of the animals in experimental groups. Muscle and nerve histology analysis are still ongoing. Our preliminary results indicate that the best outcomes are achieved when full plasma is included in the perfusate and when by-products of the perfusion are effectively filtered. Having said that, these results are still preliminary and needs further analysis once muscle and nerve histology data are available. Although we just passed the first-year mark since the official start date, we were unable to start experiments on time due to COVID restrictions which resulted in 3 months of delay. We are currently right on track to meet our deadlines as stated in SOW document for 9 months.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2021
- Accession Number
- AD1164110
Entities
People
- Kagan Ozer
Organizations
- University of Michigan