Novel Tumor Suppressor Gene in Hereditary X-Linked Ovarian Cancers
Abstract
Our previous work has identified an ovarian cancer risk locus on the X-chromosome and likelywithin the gene MAGEC3. The overall goal of this proposal is forward the idea that MAGEC3 isa tumor suppressor gene and to determine the scope and impact of the mechanism. Aim 1 isfocused on studying the mechanism of MAGEC3 silencing in clinical samples. Aim 2 uses celllines to study the function of MAGEC3 and Aim 3 will confirm our findings in mouse xenograftmodels. We determined that candidate tumor suppressor MAGEC3 is highly likely to betransiently expressed and cell cycle regulated with tight epigenetically-related expressionof the protein increasing the confidence that it is a tumor suppressor gene. This result wasachieved through the engineering of multiple cell lines with inducible MAGEC3 expression andtransgene tags that will enable continuing RNA and protein level analyses. Single cell levelexpression analyses confirmed cell cycle association and flow cytometry protein-levelanalysis also supports these findings.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2021
- Accession Number
- AD1168117
Entities
People
- Kevin H Eng
Organizations
- Health Research, Incorporated