Reinnervation of Paralyzed Limb Muscle by Nerve-Muscle-Endplate Grafting Technique
Abstract
In our previous annual report, we presented outcomes of the NMEG-NMZ technique and standard nerve-end-to-end anastomosis (EEA; technique control) for limb reinnervation. Overall, the NMEG-NMZ (NN) resulted in better functional recovery (79% of the control) compared to the EEA (51%). Here, we summarize the data obtained from animals in the Imm-NN/ENF, Imm-NN/ES, and Imm-ANG (technique control) groups. Three months after treatment, outcomes were evaluated by measuring toe spread distances and muscle force, quantifying the extent of axonal regeneration in the target muscle, determining the percentage of reinnervated motor endplates (MEPs),measuring muscle weight and examining fiber-type distribution. Our results showed that Imm-NN/ENF and ImmNN/ES resulted in more optimal functional recovery (90% and 85%, respectively) as compared with NMEG-NMZ alone (79%) and Imm-ANG (46%). The results confirm that the adjunctive therapies ENF and ES have the potential for improving the outcomes of the NMEG-NMZ surgery in the rat immediate limb reinnervation model.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2022
- Accession Number
- AD1168870
Entities
People
- Jingming Chen
- Li Jing
- Liancai Mu
- Stanislaw Sobotka
- Themba Nyirenda
Organizations
- Hackensack University Medical Center