Translational Targets of Ribosomal Protein RPL13 as Novel Cardiac Drivers of Differentiation in Drosophila and Human iPSCs: Implications for CHD
Abstract
There is a need to identify novel genetic networks and pathways driving Congenital Heart Disease (CHD). Our research is aimed at utilizing an unconventional gene involved in translation, the large ribosomal subunit RpL13, to extract and identify novel players and mechanisms in heart development with implications for CHD. During year one, we performed preliminary staining of fly embryos using various cardiac markers and consistent with our hypothesis, we observed changes in the proportion of cell types, suggesting that cells are undergoing cell fate switches. We therefore refined our single-cell RNAseq protocol to collect and enrich for fly cardioblasts from controls and RpL13knockdown flies and set parameters for FACS sorting. We created 10X Genomic libraries which are currently being sequenced. We will analyze the data as soon as we receive them. While we have not been able to move forward with experiments in human Multipotent Cardiac Progenitors due to lack of access, we have recently secured a new source for these cells and are excited to perform transcriptomic and proteomic analysis on these cells. We have also come up with an alternative approach that develops a new genetic tool in Drosophila that will enable us to capture the translatome with up to single-cell resolution. This would allow for better comparisons between transcriptomics and translatomic changes in fly cardio blasts.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2022
- Accession Number
- AD1170067
Entities
People
- Analyne M. Schroeder