Identification of Receptors for TDP-43 That Mediate Cellular Uptake and Neurotoxicity
Abstract
The purpose of this project is to 1) identify receptors at the cell surface that mediate entry of pathological TDP-43 protein aggregates into the cell and 2) determine the role of these receptors in the subsequently observed neurotoxicity of these TDP43 protein aggregates. This will be accomplished through the use of two varied but complementary techniques: CRISPR interference screening and a novel microenvironment mapping technique (MicroMap). Top hits will be confirmed and further investigated using biochemical and cell biological techniques. During the period of time covered by this report, we have obtained a number of crucial reagents, established and/or made more efficient a number of required techniques, and sought regulatory approval for a subset of the required experiments. These efforts have put us in a position to more efficiently complete the experiments outlined in the approved Statement of Work.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2022
- Accession Number
- AD1171243
Entities
People
- Robert C. Mercer
Organizations
- Boston University Medical Campus