Can Altered Bioenergetics Drive Antibiotic Persistence in Low-Oxygen Conditions?
Abstract
he goal of this project is to engineer genetically encoded fluorescent biosensors, which can be imaged in low-oxygen (or fully anaerobic) conditions; and apply these sensors to examine how ATP levels differ in aerobic vs. low-oxygen conditions for cells treated with antibiotics. The current toolbox of genetically encoded sensors requires oxygen to produce fluorescence. To address this issue, we will develop our sensors based on flavin-binding fluorescent proteins, which do not require oxygen to produce light. Our work will therefore establish a new sensing technique for basic research in anaerobic living systems and potentially yield new insights on how ATP correlates with antibiotic action in model bacteria.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2022
- Accession Number
- AD1172654
Entities
People
- Arnab Mukherjee
Organizations
- University of California Regents