Wearable Biosensors for Real-Time Physiological Monitoring
Abstract
This project builds on a novel sensor platform which takes the enzyme from glucometers (PQQGDH), and uses synthetic biology to re-engineer it into a biosensor that requires a biomarker of choice for function. This provides sensitive and specific detection of biomarkers suitable for continuous electrochemical readout. Although pandemic related delays and a death of one of the Chief Investigators have impacted the project, we have made substantial progress. Work has centered on construction of biosensor electrodes first by entrapping developed biosensors through dialysis membrane entrapment, and subsequently employing orientated immobilization and performance evaluation of the PQQ-GDH functionalized electrodes. Performance of the developed biosensors was tested in biofluids. Most recently, modified sensor architecture using caged peptide and a switchable luminescent/fluorescent readout was developed. Our work has identified the rate of GDH biosensor activation as the critical parameter for its suitability for continuous monitoring.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2021
- Accession Number
- AD1172655
Entities
People
- Artem Melman
- Evgeny Katz
- Kiril Alexandrov
Organizations
- Clarkson University
- University of Queensland