Development of the Anti-inflammatory Mast Cell
Abstract
To develop an anti-inflammatory Mast cell incapable of generating an allergic response, we are working towards knocking out key genes required by Mast cells to mediate the allergic response. To develop stably transformed Mast cells, we initially testedlipofectamine3000 and nucleofection (DS150). The Mast cells are sensitive to both and there was substantial cell death. While we do have transformed cells, we are currently exploring using a lentivirus approach for future transfections and testing whether this approach leads to higher yields of transformed cells. Currently we are testing cells in which histidine decarboxylase (HDC) gene has been knockout out which will prevent the formation of histamine (year one goals). We are expanding and testing the transfected cells for stability and once we have expanded a stable transfected line of cells, we will then test the transfected Mast cells in functional assays to determine if histamine is not produced upon Mast cell degranulation. Our next steps are to continue knocking out key Mast cell genes by targeting cyclooxygenase 1 (COX-1, year 2 goals).
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2022
- Accession Number
- AD1172672
Entities
People
- Mary Premenko-lanier
Organizations
- SRI International