Growth and/or Recruitment of a Novel Cell Population with Neural Crest Origin in Lung Fibrosis
Abstract
Pulmonary fibrosis is a debilitating disease characterized by progressive scarring of the lung, which destroys normal lungstructure and leads to respiratory failure and death Abnormally increased fibroblasts/myofibroblasts is one of the key pathologicalchanges in lung fibrosis. The origins of these abnormal lung fibroblasts/myofibroblasts are highly heterogeneous. In this project,we plan to determine whether abnormal growth and/or recruitment of a neural crest derived mesenchymal cell populationcontribute to lung fibrosis. In the past year, we have generated a transgenic reporter mouse line in which neural crest-derivedcells were genetically labeled. In normal situation, neural crest-derived cells were detected as nerve fibers adjacent to airwaysmooth muscles. In contrast, in some bleomycin-induced fibrosis lungs, clusters of neural crest derived cells were detected. Thisabnormal cellular phenotype varied, and the potential factors affecting this change are currently under investigation. In addition,circulating neural crest-derived mesenchymal progenitor cells were detected in a few fibrosis mice by peripheral bloodmononuclear cell isolation and selective culture. These cultured cells, which were negative for epithelium-marker, were MSClike progenitors and able to differentiate to other type cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2022
- Accession Number
- AD1173403
Entities
People
- Wei Shi
Organizations
- Children's Hospital Los Angeles