Molecular Cross-Talk: Bone Metastatic Prostate Cancer and Nociceptive Neurons
Abstract
The goal of this project is to determine the roles of angiotensin II and its receptor in prostate cancer induced bone pain and bone metastatic growth. Aim 1 will provide the framework to identify the extent to which the interaction between cancer cells and nociceptive neurons through the angiotensin II and receptor axis affects cancer-induced bone pain. Aim 2 will determine the downstream molecular mechanisms whereby angiotensin II and its receptor axis affect bone pain. Aim 3 will define how nociceptive neuron influence tumor outgrowth. We believe that the insights derived from our investigations will lead to new strategies for reducing cancer-induced bone pain and also the outgrowth of bone metastasis. During this period, we further identified the roles of cancer-derived angiotensin Il in the nerve growth in the bone metastatic setting. Additionally, we elucidated the crosstalk between prostate cancer cells and sensory neurons in vitro. We also found that global deletion of calcitonin gene-related peptide (CGRP) did not affect bone metastatic progression of prostate cancer. We generated and validated an RM1-mCherry cell line for use in future studies examining the relationship between bone cancer pain and plasticity of subpopulations of sensory neurons using several transgenic reporter mice and deep tissue clearing and light sheet and multi-photon confocal imaging techniques.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 05, 2022
- Accession Number
- AD1174543
Entities
People
- Christopher Peters
- Yusuke Shiozawa
Organizations
- Wake Forest University