Evaluation of Adipose-Derived Stem Cells and Metformin Combination Therapy for Radiation Fibrosis
Abstract
Radiation fibrosis (RF) is a long-term side effect of either therapeutic or accidental exposure to radiations. There is currently no therapy for treatment of RF. Through execution of our proposed work, we are trying to develop a therapy approach using a combination of adipose derived stem cells (ASCs) and metformin. We are testing the dose and time of the therapy and in addition testing the use of allogeneic ASCs as a mitigator for mass exposure scenarios. Data from our ongoing studies indicated that 3 million dose of ASCs showed better mitigation compared to 1 million cells. Allogeneic ASCs showed promising mitigation of RF and can be further evaluated for adaptation to countermeasure reserves. Combination of ASCs and metformin showed better mitigation compared to alone use of these agents for acute RF but is not in the significant range. Ongoing experiments will help to reach a more concrete conclusion. Mechanistic studies reveal no side effects of metformin treatment on ASCs rather metformin improves the health of ASCs by reducing oxidative stress and pro-oncogenic signaling.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2022
- Accession Number
- AD1174565
Entities
People
- Asim Ejaz
Organizations
- University of Pittsburgh