Inflammation and the Malignant Transformation of Endometriosis
Abstract
We conducted a study to examine the association between inflammatory markers in peritoneal fluid and driver mutations and markers of cell proliferation and invasiveness in endometriosis tissue. We will also evaluated whether inflammation-related epidemiologic factors and systemic inflammation (e.g., CRP, IL-6 plasma levels) are associated with inflammatory markers in peritoneal fluid. Data and specimens for this study have been previously collected from A2A cohort, a longitudinal cohort of women oversampled for those surgically diagnosed with endometriosis. This project consists of selecting appropriate individuals with peritoneal fluid, endometriosis tissue, blood, and epidemiologic data, accessing samples for biospecimen assays, and conducting analyses. Targeted sequencing was used to identify our genes of interest, ELISA was used to measure inflammatory biomarkers in peritoneal fluid and blood, and RNA Seq to assess the tissue expression of selected markers. In our final analytic sample sets we had 52 endometriosis tissue samples all from superficial peritoneal endometriosis, and 101 samples in our inflammation relate exposures analysis. We observed a similar recurring variant in three samples (5UTR in PTEN) and additionally observed that mutational load was associated with peritoneal fluid inflammatory markers. Further, inflammatory markers in peritoneal fluid were significantly correlated with each other, indicating an overall pro-inflammatory environment in some participants. There were limited associations with inflammatory related exposures and inflammatory markers, however, there was the suggestion of lower levels of inflammation among those with more frequent analgesic use.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2022
- Accession Number
- AD1177757
Entities
People
- Holly R Harris
Organizations
- Fred Hutchinson Cancer Center