Collaborative Regulation of Translational Targets by the ARF and NF1 Tumor Suppressors
Abstract
The regulation of mRNA loading onto polysomes is an understudied area of NF1 tumor cell biology and we are poised to take advantage of the model system provided by Nf1/Arf-deficient mouse Schwann cells. This will allow us to identify which mRNAs are associated with polysomes to understand the mechanism of how mRNAs are preferentially loaded onto or unloaded from ribosomes in cells lacking Nf1 and Arf. There were two major milestones for this first year of the grant proposal: 1) isolate and maintain murine Schwann cell cultures from our mouse lines, and 2) identify mRNAs whose translation rates are lower or higher in each Schwann cell background. We have successfully been breeding colonies of Nf1fl/fl, Arffl/fl, and Nf1fl/fl/Arffl/fl mice. From these mice, we have harvested embryonic dorsal root neurosphere cells and cultured them into Schwann cells. Ribosome protection and RNA sequencing revealed 27-downregulated mRNAs and 41-upregulated mRNAs in Nf1fl/fl/Arffl/fl Schwann cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2022
- Accession Number
- AD1178548
Entities
People
- Jason D Weber
Organizations
- Washington University in St. Louis