Evaluation of Factors Associated with Increased Plasmodium falciparum Gametocyte-Specific Transcripts in Two Populations from Sub-Saharan Africa
Abstract
Malaria is a major cause of morbidity and mortality in sub-Saharan Africa due to the high prevalence of Plasmodium falciparum in that region. Molecular methods for malaria detection can be useful in identifying individuals with sub-microscopic infectionswho may be transmitting malaria. To identify individuals with sub-microscopic gametocytemias, we developed a panel of qPCR assays that quantify gametocyte stage specific RNA transcripts. These assays were validated using both highly synchronizedcultured gametocytes and a small subset of field samples before being used to characterize gametocyte carriage in two larger studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 12, 2019
- Accession Number
- AD1181545
Entities
People
- Deborah Stiffler
Organizations
- Uniformed Services University of the Health Sciences