Characterization of Heterogeneity in Biofilm Properties Among Diverse Enterococcus Faecalis Strains

Abstract

It is unclear how biofilms of Enterococcus faecalis strains may vary in parameters such as development and composition. We hypothesized that differences in biofilms exist among distinct E. faecalis strains. In this work, we evaluated in vitro biofilm formation and matrix characteristics of a panel of five genetically diverse E. faecalis lab-adapted strains and clinical isolates. All strains formed biofilms similarly when grown in TSB without dextrose (TSB-D), 90% TSB-D, or 90% TSB-D + 10% FBS. However, DMEM with10% FBS restricted the accumulation of an appreciable amount of biofilm biomass for all strains except VA1128

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Document Details

Document Type
Technical Report
Publication Date
Jun 14, 2021
Accession Number
AD1186132

Entities

People

  • Scott D. Schaffer

Organizations

  • Uniformed Services University of the Health Sciences

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacteria
  • Bacteriology
  • Cellular Structures
  • Chemistry
  • Confocal Microscopy
  • Epidemiology
  • Health Services
  • Indicator Dyes
  • Infection
  • Infection Control
  • Macrophages
  • Microbiology
  • Microbiomes
  • Pathogenic Bacteria
  • Wound Infections

Fields of Study

  • Biology
  • Environmental science

Readers

  • Immunology
  • Mathematics or Statistics
  • Microbial Pathology

Technology Areas

  • Biotechnology