8.1 Biochemistry: A Polyvalent, Allosteric Whole Virus Binding Platform for Norovirus Detection

Abstract

Major Goals: Goals: synthesis and optimization of a polyvalent DNA origami aptamer-displaying "Claw" that can be incorporated into a rapid, point-of-care, electrochemical Norovirus sensor. The proposal's aims are i) to screen various tethered/blocked claw designs to improve 1:1 capsid solution-phase binding efficiency and selectivity, ii) to functionalize surfaces with untethered claw and test binding for models of sensor construction, and iii) optimize claw aptamer recognition sites (before and after attachment to claw) to optimize binding strength and, ultimately, selectivity between different norovirus serotypes.

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Document Details

Document Type
Technical Report
Publication Date
Jan 14, 2020
Accession Number
AD1186627

Entities

People

  • Philip S. Lukeman

Tags

DTIC Thesaurus Topics

  • Attachment
  • Biosensors
  • Chemistry
  • Detection
  • Detectors
  • Dna Nanotechnology
  • Electrochemical Cells
  • Electrodes
  • Electron Transfer
  • Gel Electrophoresis
  • Materials
  • Molecular Dynamics
  • Nanotechnology
  • New York
  • Point-Of-Care Diagnostic Testing
  • Simulations
  • Students
  • Triangles
  • Universities

Readers

  • Infectious Disease/Epidemiology
  • Molecular and Cellular Biochemistry
  • Nanoscale Plasmonic Nanotechnology