Ecotoxicity of New Film-Forming Foams: Acute and Subacute Toxicity Tests in Mus musculus
Abstract
This Technical Report describes short term toxicity in mice for seven per-and polyfluoroalkyl substances (PFAS)-free and PFAS-containing aqueous film forming foam (AFFF) products under consideration for use by the Department of Defense. CD-1 tm mice are an outbred species of mice (Mus musculus) that provide some insight towards terrestrial mammalian wildlife toxicity as well as human toxicity. Exposure was via oral gavage to products as the concentrates as they are manufactured and sold prior to use at 3% dilution formulations. Exposures were acute (limit test) or subacute (28d test) with the acute test based on a single dose and the subacute test as 28 daily doses. Observations in bodyweight, tissue weights, hematological parameters, serum clinical chemistry, thyroid hormone concentration, and micronucleus assay (DNA damage) were interpreted to inform an overall perspective of short-term exposure toxicity in mice. Notably, liver weight was the most informative endpoint for comparison across AFFF products with the PFAS-containing AFFF Buckeye being the most toxic. Among the PFAS-free AFFF, NRL 502W shows the greatest toxicity with anemia and liver effects. NFD 20-391 shows the least toxicity among the PFAS-free AFFF products.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2022
- Accession Number
- AD1187035
Entities
People
- Allison M. Narizzano
- Andrew G. East
- Lindsay A. Holden
- Michael J. Jr Quinn
Organizations
- United States Army Center for Health Promotion and Preventive Medicine