Mechanisms of Extracellular Matrix-Mediated Electrochemical Signaling
Abstract
The overall aim of this project was to test the hypothesis that biofilms use the extracellular matrix as a conduit to target electrochemical signals to specific subsets of cells. To accomplish this, we proposed the following Specific Objectives: 1) Quantitatively characterize matrix pattern formation in biofilms; and 2) Establish a causal link between matrix patterns and electrochemical signaling. In previous reports, we reported methods to measure the spatial distribution of extracellular matrix in Bacillus subtilis biofilms during development (Objective 1). Then, we reported the discovery of unexpected dynamics in the extracellular DNA (eDNA) component of the matrix during biofilm development (Objective 2). These results are currently being prepared for publication. Here, in the final progress report, we describe the remarkable discovery of endogenous acetylcholine signaling as both a potential underlying source, and electrochemical mediator of, eDNA dynamics during biofilm development. These unexpected results formed the basis of a successful ECASE-Army proposal.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 31, 2022
- Accession Number
- AD1190216
Entities
People
- Arthur Prindle
Organizations
- Northwestern University