Role of AR-Derived Circular RNA in Prostate Cancer
Abstract
The androgen receptor is a key therapeutic target in prostate cancer. Multiple androgen receptor alterations are known to affect prostate cancer progression and treatment efficacy. In this proposal, we will focus on a novel form of non-coding circular RNA originated from the androgen receptor gene. We will test the hypothesis that AR-derived, non-coding circular RNAs (circARs) can act as competitive endogenous RNAs through sponging micro RNA (miRNA), or RNA-binding proteins to regulate prostate cancer progression. To this end, we proposed three Specific Aims. Aim 1 will identify and validate circARs in castration resistance prostate cancer (CRPC). Aim 2 will define the functional roles of circARs in CRPC. Aim 3 will determine the regulatory factors involved in circAR generation. During Years 1 and 2 of the funding period, we successfully initiated the study despite the limitations and challenges posed by the pandemic. All regulatory documents are now in compliance with the latest regulations. We established and validated the methodology to enrich AR transcripts for identifying circular ARs by RNAseq in prostate cancer cell lines. We have generated preliminary data. We are in the process of expanding our established method to prostate cancer patient tissues. We expect to report the main findings in the ensuing phase of the project period.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2022
- Accession Number
- AD1190401
Entities
People
- Changxue Lu
Organizations
- Johns Hopkins University