Novel Dried Cryoprecipitate-Based Intervention to Improve Outcomes from Trauma and Hemorrhagic Shock: Applicability for Multidomain Operations

Abstract

The major objective of this effort is to define the role of a five day shelf life pathogen reduced cryoprecipitate (5PRC) and lyophilized cryoprecipitate (LPRC) using 2 different small animal models of hemorrhagic shock (HS) to examine the effect of these novel products on both hemostasis and endothelial protection. The long-term goal is FDA approval of these products for clinical and field use after HS. We HYPOTHESIZE that pathogen reduced cryoprecipitate-based interventions will decrease both early hemorrhagic deaths and later MOF through their dual effects on hemostasis and endothelial stability. To test this hypothesis, the early use of LPRC and 5PRC will be compared to conventional cryoprecipitate (CC),fresh frozen plasma (FFP) and standard of care Lactated Ringers (LR) in two different rodent models of trauma/HS applicable for multi-domain operations in the following specific aims: SA1. Determine the effect of early cryoprecipitate on hemostasis, organ function, and mortality in a short term mouse model of trauma and uncontrolled hemorrhage (UCH). A well-established mouse liver transection model of UCH will be utilized followed by resuscitation with the described blood products or LR and compared to sham animals. Hemostasis, coagulation, lung function, endothelial integrity and short-term mortality will be assessed. SA2. Determine the effect of early cryoprecipitate on endothelial protection, organ failure and mortality in a mouse model of sustained hypotensive resuscitation. Our established mouse model of trauma/HS and sustained hypotensive resuscitation with the described blood products or LR and compared to shams. Following pronged hypotensive resuscitation (PHR) animals will be followed for 2 days to track survival.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2022
Accession Number
AD1190509

Entities

People

  • Rosemary Kozar

Organizations

  • University of Maryland, Baltimore

Tags

DTIC Thesaurus Topics

  • Arteries
  • Biomedical Research
  • Blood
  • Blood Pressure
  • Data Analysis
  • Endothelial Cells
  • Governments
  • Hemorrhagic Shock
  • Hemostasis
  • Histology
  • History
  • Humanities
  • Intervention
  • Language
  • Local Governments
  • Maryland
  • Medical Personnel
  • Patent Applications
  • Professional Development
  • Shelf Life
  • Students
  • Training
  • Wounds And Injuries

Fields of Study

  • Medicine

Readers

  • Trauma Surgery or Emergency Medicine.