Proteomics of Knee Osteoarthritis
Abstract
MRL/MpJ (super healers) mice have a unique ability to repair wounds and are protected from cartilage degradation subsequentto joint trauma. The hypothesis is that in response to injury, MRL/MpJ mice synthesize proteins that (1) protect the joint fromcartilage degradation and/or (2) promote cartilage regeneration. The PIs propose to generate an atlas of the injury-activatedproteome in mouse models with varying susceptibility to posttraumatic osteoarthritis (PTOA): (1) C57BL/6; (2) C57BL/6treated with streptozotocin (STZ), a model of type 1 diabetes; (3) MRL/MpJ (super healers); and (4) STR/ort (spontaneousOA). By conducting comparative proteomics of injured and uninjured joints, the PIs will identify novel protein candidates forfurther exploration as potential therapies for treating injured joints. The projects specific aims are (1) application of in vivometabolic labeling to quantify and characterize de novo protein synthesis, cellular proliferation, and mineral apposition ininjured joints of mice with varying susceptibility to PTOA and (2) identification of newly synthesized RNA and proteins in thearticular cartilage and immune cells of injured knees using a liquid sample interface for the AMS instrument in combinationwith liquid chromatography-mass spectrometry (LC-MS).
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2022
- Accession Number
- AD1190853
Entities
People
- Blaine A Christiansen
- Gabriela G Loots
Organizations
- University of California