Quantifying In Situ Adaptive Immunity in Human Tubulointerstitial Lupus Nephritis
Abstract
The specific goals of this grant are to use high-dimensional (16 primary antibodies or more) multicolorconfocal microscopy to describe the frequency and spatial distributions of the different types of immunecells infiltrating the lupus kidney (Aim 1). We will then use machine learning approaches, including CDM3,to quantify the true complexity of inflammation and identify functional relationships between different T cellpopulations and the cells that are presenting antigen, and therefore activating, pathogenic T cells (Aim 2).By understanding which cells, functional relationships and mechanisms are associated with progression torenal failure, we will both identify new therapeutic targets and the patients for which such therapies arelikely to be efficacious.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2022
- Accession Number
- AD1191042
Entities
People
- Marcus R Clark
Organizations
- University of Chicago