Multiplex Functional Interrogation of Combinations of Genetic Alterations in Prostate Cancer
Abstract
During the grant period, we have developed a higher-order, combinatorial genetics strategy using lentiviral (LV) libraries of cancer-associated genetic events introduced into benign epithelial cells that are subsequently engrafted in vivo for tumorigenic selection. We have generated several tumors using this approach that demonstrate clinically relevant, mixed cancer histologies. We incorporated massively parallel single-cell LV barcode sequencing to identify genetic alterations that contribute to the diverse phenotypes represented in the tumors. Validation of the findings is currently ongoing. These initial studies provide proof-of-principle of this powerful approach to rapidly investigate genotype-phenotype relationships in cancer initiation starting from primary epithelial cells. Our ultimate goal is to generate genetically defined models of prostate cancer that recapitulate the diversity of human prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2022
- Accession Number
- AD1191478
Entities
People
- Shan Li
Organizations
- Fred Hutchinson Cancer Center