Mechanical Properties of the Injured CNS: Implications for Remyelination and Axonal Repair
Abstract
This research provided novel insights regarding micro-scale changes in the mechanical properties of acute and chronic demyelinating brain lesions, and how these changes might correlate to remyelination failure in advance stages of MS. We found that changes in matrix stiffness can impact the differentiation of iPSC-derived human glial lineages, and that stiffer matrices promotes astrocyte differentiation over that of oligodendrocytes. Preliminary findings indicate that the subcellular localization of the mechanosensitive transcription factor YAP in oligodendrocytes can be predictive of their ability to differentiative. Specifically, retention of nuclear YAP is negatively correlated with myelin protein expression at the single cell level in myelinating co-cultures. These findings have the potential to help optimize the methods currently used to generate human oligodendrocytes for autologous transplantation using iPSC technology, as well as guide the efforts for the efficient targeting and delivery of these cells to MS patients.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2022
- Accession Number
- AD1193637
Entities
People
- Carmen Melendez-Vasquez
Organizations
- City University of New York
- Hunter College