Single Molecule DNA Origami Nanoarrays with Controlled Protein Orientation
Abstract
The nanoscale organization of functional (bio)molecules on solid substrates with nanoscale spatial resolution and singlemoleculecontrolin both position and orientationis of great interest for the development of next-generation (bio)moleculardevices and assays. Herein, we report the fabrication of nanoarrays of individual proteins (and dyes) via the selectiveorganization of DNA origami on nanopatterned surfaces and with controlled protein orientation. Nanoapertures in metalcoatedglass substrates were patterned using focused ion beam lithography; 88 of the nanoapertures allowed immobilizationof functionalized DNA origami structures. Photobleaching experiments of dye-functionalized DNA nanostructures indicatedthat 85 of the nanoapertures contain a single origami unit, with only 3 exhibiting double occupancy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 21, 2022
- Accession Number
- AD1194281
Entities
People
- D. D. Jones
- Jorge L. Chávez
- K Cervantes-salguero
- M Palma
- M. Freeley
- R E Gwyther
Organizations
- Queen Mary University of London