SERS Nanosensors for in Vivo Glucose Sensing
Abstract
The goal of this program was to develop small and sensitive nanosensors for the continuous glucose monitoring in living tissue without the need for drawing blood. A major advantage of the transdermal sensors we are developing is to directly detect glucose itself not the byproducts of its transformation. The technique we use surface-enhanced Raman spectroscopy (SERS) is based on light and informs on the presence of glucose on or near metallic nanosensors. In Major Task 1, we worked on the development of i) sensitive nanosensors, ii) selective capture layers that can be immobilized onto metal surfaces, iii) the integration of ligands on nanoparticles, and iv) integration of the functionalized nanoparticles with microneedle arrays for SERS sensing. We have successfully developed novel SERS nanoplatforms that integrates gold nanoparticles and capture layers. We have also developed electro-chemical SERS methods, integrated with microneedle arrays, to sense biomolecules without the use of a small molecule binding ligand. In Major Task 2, we evaluated the biocompatibility and mechanical stability of functionalized plasmonic SERS microarray sensors and demonstrated their ability to measure pH in skin phantom models after repeated implantations. We also evaluated thermal stability. In Major Task 3, the SERS performance of the plasmonic microneedle arrays were evaluated ex vivo in rat, pig, and human skin samples.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2022
- Accession Number
- AD1194713
Entities
People
- Eric Berns
- Guru Neupane
- Ji E. Park
- Milan Mrksich
- Vitor Brasiliense
Organizations
- Northwestern University