Role of SCARF1 in Efferocytosis and Prevention of Lupus
Abstract
During the first year of funding, we made substantial progress in characterizing the role of SCARF1 in hematopoietic vs nonhematopoietic cells in the development of lupus-like disease on our mouse model. This will help up identifying a group of cells that are essential in the development of lupus-like disease. We hypothesized that hematopoietic cells are essential in the development of disease and mice that lack SCARF1 in dendritic cells and macrophages will develop lupus like symptoms. We perform chimera experiments using the following groups: WT->WT, SCARF1->SCARF1 as our controls, and SCARF1->WT, WT->SCARF1 as experimental samples. While the experiment still on going, our initial findings show that WT mice that have SCARF1-/- cells developed dermatitis, the production of ANA and release of dsDNA. Presentation of symptoms was faster, with more pronounced symptoms in the SCARF1->WT group compared to the WT->SCARF1 group. However, WT->SCARF1 mice group show mild lupus like disease suggesting that the non-hematopoietic cells also play a role disease development. Our data suggest that hematopoietic cells (DCs, macrophages, monocytes) are essential in the initiation of SLE; however, non-hematopoietic could play a role in the maintaining of the disease.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2022
- Accession Number
- AD1196246
Entities
People
- Zaida Ramirez-ortiz
Organizations
- University of Massachusetts