Airway Reconstruction via Stem Cell-Based Therapy
Abstract
Airway damages in the combat zone is among the leading causes of potentially preventable combat death. While the reasons for the high degree of tissue loss and destruction are multifactorial, i.e. improvised explosive devices and toxic gas exposure, the consequences could be devastating and lethal. With these acute trauma patients in mind, the goal of this project is to create a bioengineered tracheal/airway graft with functional epithelium using stem cell-based approaches. This past year was the second year of this funding award and the relocation of the laboratory, and the award transfer impacted our research activities in a negative way significantly. Despite the challenges and unusual circumstances, we still made progress and optimized the de-epithelialization condition.We also tested two different recellularization methods and found one of the methods gave us approximately 25% of human cell coverage on the de-epithelialized tracheas 1 day post seeding and about 70% of coverage 3 days post seeding. During the next reporting period, we hope to make up the lost time and effort toward completion of the proposed research.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2023
- Accession Number
- AD1196924
Entities
People
- Beiyun Zhou
- Ya-Wen Chen
Organizations
- Icahn School of Medicine at Mount Sinai
- University of Southern California