DNA-Mediated Proximity Assembly Circuits for Point-of-Care Diagnosis
Abstract
We proposed to commercialize a DNA nanodevice product that can be adopted for POC testing.The nanodevice uses a mechanism of DNA-mediated proximity assembly circuit (DPAC) that contains a limited number of oligonucleotides and a simple catalytic system. Target molecules can be identified by visible color change. It does not require any additional steps to separate detectable components from the assay background,and can be applied to at-home use or diagnosis at diverse locations. Our developed DPAC sensors have the advantages: (1) simplified operation (e.g. One-pot assay) by non-specialists at diverse locations; (2) easy-to-read signals; (3) reliable and rapid assay; (4) low-cost for the test. As proof-of-concept, Dr. Fu's team has developed and tested a DPAC sensor to detect a microRNA biomarker for prostate cancer at concentration as low as 0.1 nM.Similar sensors can be readily redesigned for the detection of other disease-relevant nucleic acids, proteins and small molecules that have broad use in healthcare, environmental monitoring and safety of soldiers at ground warfare. The target market includes: POC testing for a variety of disease-relevant nucleic acids, small molecules and proteins; metal detection in water; detection of reactional drug metabolites and explosive compounds sensing.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 25, 2022
- Accession Number
- AD1198784
Entities
People
- Bo Wang
- Jinglin Fu
- Kristin Monckton
- Sung W Oh
Organizations
- Rutgers University–Newark