Multiplex Functional Interrogation of Oncogenic Networks in Bladder Cancer
Abstract
We have developed and characterized a new methodology to generate genotypically and phenotypically diverse mouse models of bladder cancer that may better reflect the heterogeneity of the human disease. We have introduced lentiviral pools encoding gain- and loss-of-function events associated with bladder cancer in multiplexed fashion in the context of a mouse bladder urothelial transformation assay. This led to the generation of multiple tumors models that are polyclonal and exhibit multiple histologies including typical urothelial carcinoma, papillary urothelial carcinoma, squamous cell carcinoma, and sarcomatoid carcinoma. Furthermore, transcriptional profiling studies and the application of published molecular classifiers indicate that these tumors reflect multiple molecular subtypes of bladder cancer. This methodology and the resultant tumor models may be a valuable resource for the field in understanding the interactions of oncogenic events in tumorigenesis and investigating therapeutic strategies in bladder cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2022
- Accession Number
- AD1199174
Entities
People
- John K Lee
Organizations
- Fred Hutchinson Cancer Center