In Vivo and Ex Vivo Models to Study Ischemia/Reperfusion Injury, Endothelial Cell Protection, and Limb Preservation in a Prolonged Field Care Scenario
Abstract
In this project, endothelial cell protection and extracorporeal machine perfusion will be combined to achieve a massive prolongation of the time between traumatic amputation and replantation. In the third year, all animal experiments for Milestone 2: 'Validation of ex vivo in vivo models for the effect of EC protection on extremity I/R injury in a PFC scenario' were performed, as well as most of the respective laboratory analyses. We found that the use of the endothelial cell protectant C1-INH led to reduced histological tissue damage in the reperfused limbs, both ex vivo and in vivo after replantation. However, this did not result in less weight increase or a lower wet/dry ratio of ex vivo reperfused limbs. In addition, we could show that replantation and in vivo reperfusion of a 9h ischemic limb led to remote organ damage of lungs, kidney and liver, which was clearly visible by classical HE-staining as well as by immunofluorescence analysis for complement deposition. This project was granted a cost-neutral 12-monthextension, and all aims as set out in the original SOW will be reached until 08/23.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2022
- Accession Number
- AD1199717
Entities
People
- Robert Rieben
Organizations
- University of Bern