Development of a Multifaceted Telemetry System for Physiological Data Collection in Ferrets
Abstract
When investigating pathophysiological responses to novel chemical toxicants, describing homeostasis of major vital organ systems is critical to understanding hosttoxicant interaction. In this study, we developed a ferret model to collect data that describe the cardiovascular (electrocardiogram and blood pressure), central nervous system (electroencephalogram), and respiratory system responses (diaphragmatic electromyogram, dEMG) as well as body temperature and animal activity. In addition, a jugular vein cannula attached to a vascular access button was inserted in each ferret for collecting serial blood samples. We used two systems of telemetry units. The first system consisted of one each of HD-X02 and HD-S11-F2 devices. This system was found to be less than ideal. The second system consisted of one each of F50-EEE and HD-S1-F2 devices. It was found to be more compatible with the regular housing and experimental conditions utilized for ferrets. A total of 18 ferrets were tested; 17 of them survived the surgical procedures. Herein we detail the optimized surgical procedures and housing arrangements and provide example data acquired from the implanted animals. Following this study, a more in-depth investigation into the utility of the dEMG for derivation of volume-related respiratory parameters will be conducted.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 11, 2023
- Accession Number
- AD1201214
Entities
People
- Chelsea R. Boeri
- Michael S. Horsmon