mRNA Vaccine for Peanut Allergy
Abstract
During Year 1 of this Discovery Award, we have made significant progress on Specific Aim 1. We provided peanut allergen sequence information to Synbio Technologies who then synthesized Ara h 1, 2, 3 and 6 mRNA, as well as mouse IL-12. We transfected HEK 293T cells with the Ara h 1-6 mRNAs and confirmed protein expression by Western Blot with plasma from peanut-allergic subjects. Cell supernatants and lysates have been sent to MS Bioworks for mass spectrometry analysis of protein expression levels. Once the protein expression has been confirmed by mass spectrometry, we will formulate the mRNA in lipid nanoparticles for intramuscular delivery in mice, and we will send the mRNA to Orlance for formulation on gold microbeads for Gene Gun delivery in mice. Overall, our data demonstrates that peanut allergen mRNA can be expressed in mammalian cells, indicating that we are making progress towards the mRNA vaccine.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2023
- Accession Number
- AD1202710
Entities
People
- Michael Kulis
Organizations
- University of North Carolina at Chapel Hill