Cholesterol Regulation in Familial Hypercholesterolemia by Long Noncoding RNA

Abstract

Familial Hypercholesterolemia (FH) is a genetic disorder within the FY21 PRMRP Topic Areas. Whether long non-coding RNAs (lncRNAs) have the therapeutic potential in FH remains unclear. In our preliminary study, we identified a lncRNA as a regulator of cholesterol metabolism and a potential therapeutic target for FH. During the research period, we have further confirm edits critical role in cholesterol metabolism in hepatocytes. Moreover, we have found that this lncRNA regulates cholesterol metabolism through the transcription factor SREBP2. To study the function of this lncRNA in vivo, we have produced adenovirus vector for overexpression of this lncRNA. We found that overexpressing this lncRNA in mouse liver suppresses the expression of SREBP2 pathway genes. Consistent with this, the liver and blood cholesterol levels were decreased. We also found that the overexpression of this lncRNA suppresses the transcription of the SREBP2 pathway genes by performing CHIP-PCR analysis.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2023
Accession Number
AD1202720

Entities

People

  • Ling Yang

Organizations

  • Temple University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Adenoviruses
  • Biomedical Research
  • Cells
  • Diseases
  • Epithelial Cells
  • Lipids
  • Metabolic Diseases
  • Metabolism
  • Pcr Testing
  • Professional Development
  • Proteins
  • Regulations
  • Regulators
  • Rna Stability
  • Students
  • Transcription Factors
  • Universities

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and Cellular Biology

Technology Areas

  • Biotechnology