Discovery of New Antimicrobial Compounds from Marine Actinobacteria Associated with Sponges

Abstract

This project looks at the diversity of antimicrobial-producing actinobacteria in sponges. Sponges are known to produce natural products such as antimicrobial compounds and are known to harbor actinobacteria which are a known source of antibiotics. In this project, we have collected six sponge samples by SCUBA diving and isolated 416 bacterial strains (203 from site 1, 134 from site 2, and 79 from site 3.) Of the strains from site 1, 33 were found to be gram positive and genetically analyzed. DNA sequencing of the partial 16s ribosomal RNA gene revealed that 17 of these 33 matched up to known actinobacteria. The DNA of all strains from sites 2 and 3 have been isolated and prepared for sequencing. Glycerol stocks of all identified actinomycetes will be preserved at -80C and made available for antimicrobial activity assays by AFRL

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Document Details

Document Type
Technical Report
Publication Date
Sep 29, 2020
Accession Number
AD1204595

Entities

People

  • Govind S. Nadathur

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Actinobacteria
  • Air Force
  • Air Force Research Laboratories
  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Aquatic Organisms
  • Bacteria
  • Bacteriology
  • Biological Products
  • Deoxyribonucleic Acids
  • Dna Sequence Analysis
  • Fungi
  • Gram-Negative Bacteria
  • Gram-Positive Bacteria
  • Microbial Genome
  • Microbiology
  • Microbiomes
  • Microorganisms
  • Oceanography
  • Prokaryotes

Fields of Study

  • Biology
  • Environmental science

Readers

  • Materials Science
  • Microbial Pathology
  • Molecular Genetics

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation