Amplification Events Altering Tumor Microenvironment That Drive Metastasis in HER2+ Breast Cancer

Abstract

In the past year a new trainee has been added to the project and we have proceeded through rounds of selection to generate the CRISPR constructs. In the generation of the knockdowns, significant issues were identified with 5 rounds of troubleshooting to eventually identify the fact that our lentiviral system was not effectively packaging virus (after switching to a newer generation lentivirus due to flow sorting facility requirements). We are now able to generate lentivirus and have generated knockdowns of the target genes (Col1A1, CHAD and PHB). While selecting for knockdowns, assays have been optimized to characterize metastatic progression and the steps at which metastasis may be blocked, from in vitro migration assays to culture of single cells from blood to examination of metastasis of parental lines in lungs as well as the lymphatic system.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2023
Accession Number
AD1206352

Entities

People

  • Eran R Andrechek

Organizations

  • Michigan State University

Tags

DTIC Thesaurus Topics

  • Amplification
  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Culture Techniques
  • Demographic Cohorts
  • Department Of Defense
  • Mammary Glands
  • Medical Personnel
  • Metastasis
  • Neoplasms
  • Sequence Analysis
  • Students
  • Tissue Culture
  • Training
  • Whole Genome Sequencing

Readers

  • Molecular Genetics
  • Oncology (Cancer Research).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech