Developing Cell-Based and Mechanism-Focused Preclinical Platforms with Diseased Upper Motor Neurons
Abstract
This is a two-year DoD grant, allowing us to develop a semi-high throughput drug discovery and verification platform that incorporates the health of diseased upper motor neurons as an outcome measure, so that the cortical component of ALS can also be incorporated into drug discovery efforts in ALS. Since, no compound in clinical trials has ever been tested for their efficacy on improving the health of diseased upper motor neurons, this proposal fills an important gap in the field. On our first year, we developed mixed cortical cultures from four different disease models, each of which recapitulate a different aspect of the disease, such as the misfolded SOD1 toxicity, TDP-43 pathology and absence of alsin and profilin function. We optimized the high throughput plate culturing, (ie number of cells, the confluency, time of culture and the mode of tile imaging) so that reproducible results can be obtained from each culture condition. The first year was important for optimization and we hope and plan to have more robust experimental output in the second year.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2023
- Accession Number
- AD1206979
Entities
People
- Hande Ozdinier
Organizations
- Northwestern University