Early Intervention Stem Cell-Based Therapy (EISCBT) for Corneal Burns and Trauma

Abstract

The goal of this project is to validate new stem cell markers which can be used to assess the corneal stromal stem cell (CSSC) regenerative potential and develop a regenerative corneal bandage (ReCoBand) with CSSC embedded in or with a regenerative extracellular matrix that can be applied in the battlefield to prevent permanent scarring of the cornea and treat the corneal scarring after trauma, blast, or burn wounds to preserve vision. In the first year of this project we have: (1) validated stem cell markers with which CSSC possess the regenerative potential, (2) characterized a transgenic mouse model which the fibrotic cells turning to green when the cornea is scarred, (3) confirmed that CSSC can prevent corneal scarring on this transgenic Col3a1-EGFP mouse model, (4)discovered one of the mechanisms that CSSC prevent corneal scar formation, (5) proved that ReCoBand with verified CSSC can prevent corneal scar formation in an alkali burn rabbit model. These accomplishments meet the proposed milestones and predict success in meeting the overall goals of the project by the end of the 2 year time course.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2022
Accession Number
AD1207384

Entities

People

  • Yiqin Du

Organizations

  • University of Pittsburgh

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Cell Line
  • Cells
  • Culture Techniques
  • Cultured Cells
  • Department Of Defense
  • Gene Expression
  • Governments
  • Humanities
  • Intervention
  • Law
  • Local Governments
  • Medical Personnel
  • Ophthalmology
  • Professional Development
  • Stem Cells
  • Students
  • United States
  • Wound Healing

Readers

  • Immunology and Pathology
  • Neurotrauma and Rehabilitation Medicine.

Technology Areas

  • Biotechnology