Reinnervation of Paralyzed Limb Muscle by Nerve-Muscle-Endplate Grafting Technique
Abstract
During the past year, we conducted experiments on animals in the 3-month delayed reinnervation groups (i.e., DelNN, Del-ENF/NN, Del-SP/NN, Del-ANG, and Del-EEA) and 6-month denervation control. Three months aftertreatment, the extent of functional recovery of the treated TA muscles was examined by measuring toe spread distances and maximal muscle force. The extent of neuromuscular recovery was examined by quantifying regenerated axons, reinnervated motor endplates (MEPs), muscle weight, and denervated/atrophied muscle fibers. Our results showed that Del-ENF/NN and Del-SP/NN resulted in better muscle force recovery (72 percent and 70 percent of the control, respectively) than Del-NN alone (59 percent), Del-ANG (38 percent) and Del-EEA (43 percent). Tissue studies showed that Del-ENF/NN and Del-SP/NN resulted in better axonal regeneration, MEP reinnervation, and muscle mass preservation as compared with other delayed reinnervation groups used in this research. These findings suggest that focal injection of ENF and SP procedure are useful therapies for delayed muscle reinnervation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2023
- Accession Number
- AD1210237
Entities
People
- Jingming Chen
- Li Jing
- Liancai Mu
- Stanislaw Sobotka
- Themba Nyirenda
Organizations
- Hackensack University Medical Center