Effects of Acute and Subacute Oral 3,4-Dinitropyrazole (DNP) Exposure to Rats(Rattus Norvegicus)
Abstract
The acute oral median lethal dose, 95 percent confidence intervals, and slope of 3,4-dinitropyrazole (DNP) in female rats were determined to be 444mg/kg, 364-542 mg/kg, and 9.77, respectively. Subacute administration of DNP in male and female rats did not result in mortality at doses up to 222 mg/kg-d with only minor, sporadic clinical signs. Body mass gain was reduced in the male 222 mg/kg--d group throughout the study. Numerous subtle, sporadic clinical pathology parameters were observed throughout all male and female DNP dose groups along with significant decreases in absolute and normalized masses of adrenals, heart, kidneys, and spleen. Despite the consistent absence of a dose response for nearly every parameter measured and the relatively minor magnitudes of effect, the rats were determined to have developed mild kidney injury and immunological abnormalities at all doses tested resulting in a lowest observed adverse effect level (LOAEL) of 14 mg/kg-d. Benchmark dose analysis was not determined due to the absence of a monotonic dose response relationship.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2023
- Accession Number
- AD1210826
Entities
People
- Allison M. Narizzano
- Lee C. Crouse
- Matthew A. Bazar
- Michael J. Quinn
- Thomas E. Sussan
- Valerie H. Adams
Organizations
- United States Army Public Health Command